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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Cell Rep. 2020 May 5;31(5):107605. doi: 10.1016/j.celrep.2020.107605

Figure 6. In Situ Hybridization and Co-Immunoprecipitation (IP) Characterize Native mGluR2/3 Co-expression and Heterodimerization.

Figure 6.

(A) Representative fluorescence in situ hybridization (FISH) for Grm2 (green) and Grm3 (red) RNA and cell delineation using DAPI (blue) in cortical cells. Scale bar, 25 µm.

(B) Bar graphs showing the percentage of cells expressing Grm2, Grm3, or both, in different brain areas.

(C and D) FISH analysis of M2/Cg (C) and PL (D) cortex with quantification of co-expression of Grm2 and Grm3 across different layers. FISH data are analyzed across three mice. Data are presented as mean ± SEM.

(E) Western blots showing that following immunoprecipitation from frontal cortex homogenate with a specific anti-mGluR3 antibody, bands can be detected for both mGluR3 (left) and mGluR2 (right). Controls using an anti-IgG antibody show no immunoprecipitation of mGluR3 or mGluR2. Representative blots are shown; the experiment was repeated three times with identical results.

M2, secondary motor cortex; Cg, cingulate cortex; PL, prelimbic cortex; IL, infralimbic cortex; NAcc, nucleus accumbens; DS, dorsal striatum; BLA, basolateral amygdala.