KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-HA tag antibody (Biotinylated) | abcam | Cat#ab6438; RRID:AB_2115899 |
| mGluR3 antibody | abcam | Cat#ab166608; RRID:AB_2833092 |
| mGluR2 antibody | abcam | Cat#ab15672; RRID:AB_302021 |
| recombinant rabbit IgG | abcam | Cat#ab172730; RRID:AB_2687931 |
| Goat Anti-Mouse IgG H&L (HRP) | abcam | Cat#ab6789; RRID:AB_955439 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| NeutrAvidin Protein | Thermo Fisher Scientific | Cat#31000 |
| benzylguanine (BG)-LD655 | Gutzeit et al., 2019 | N/A |
| SNAP-Surface Alexa Fluor 647 | New England Biolabs | Cat#S9136S |
| CLIP-Surface 547 | New England Biolabs | Cat#S9233S |
| SNAP-Surface 488 | New England Biolabs | Cat#S9232S |
| CLIP-Surface 647 | New England Biolabs | Cat#S9234S |
| SNAP-Cell TMR star | New England Biolabs | Cat#S9105s |
| mPEG | Laysan Bio | Item# BIO- PEG-SVA-5K- And MPEG- SVA- 5K |
| biotinylated mPEG | Laysan Bio | Item# BIO- PEG-SVA-5K- 100MG and MPEG-SVA- 5K |
| IGEPAL | Sigma Aldrich | I8896–50 |
| DMEM | Thermo Fisher Scientific | Cat#11995073 |
| Fetal Bovine Serum | Thermo Fisher Scientific | Cat#10437028 |
| Lipofectamine 2000 Transfection Reagent | Thermo Fisher Scientific | Cat#11668–019 |
| Poly-L-lysine hydrobromide | Sigma Aldrich | P2636 |
| Mm-Grm2-C3 | ACD/Biotechne | Cat#317831-C3 |
| Mm-Grm3-C2 | ACD/Biotechne | Cat#317821-C2 |
| Critical Commercial Assays | ||
| RNAscope Multiplex Fluorescent Reagent Kit v2 4-plex | ACD/Biotechne | Cat#323120 |
| Experimental Models: Cell Lines | ||
| HEK293T | American Type Culture Collection (ATCC) | ATCC Cat# CRL-11268 RRID: CVCL_1926 |
| Experimental Models: Organisms/Strain | ||
| Mice C57BL/6J Strain | The Jackson Laboratory | Stock No:000664 (IMSR Cat# JAX:000664, RRID:IMSR_JAX:000664) |
| Recombinant DNA | ||
| SNAP- and CLIP-tagged mGluRs | Doumazane et al., 2011 | N/A |
| GFP-tagged mGluRs | Levitz et al., 2016 | N/A |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | RRID:SCR_003070 |
| Origin | Origin Lab Corporation | RRID:SCR_002815 |
| Microsoft Excel | Microsoft Office | RRID:SCR_016137 |
| Adobe Illustrator | Adobe | RRID:SCR_010279 |
| Olympus CellSens | Olympus | RRID:SCR_016238 |
| LabVIEW | National Instruments | RRID:SCR_014325 |
| IMARIS 9.5 | Bitplane | RRID:SCR_007370 |
| RStudio | RStudio, inc. | RRID:SCR_000432 |
| IgorPro | Wavemetrics | RRID:SCR_000325 |