Figure 9.

Enzyme activity of SUS was changed in OsRePRP2-OX. A, Co-IP (IP) assays of GFP, OsRePRP1-GFP, and OsRePRP2-GFP transgenic rice. Total protein was extracted from whole roots of 4-d-old seedlings treated with 2 μm ABA for 2 d. Western blot analysis involved anti-SUS antibodies. The arrow indicates 93-kD SUS. Two biological replicates were performed. B and C, Enzyme activity of SUS in the cleavage (B) and synthetic (C) directions. Total protein extract from 14-d-old whole roots of the wild type (WT), OsRePRP2-OX (OX), and OsRePRP-Ri (Ri) is shown in box plots. The line inside the box indicates the median, and the ex indicates the mean. Bottom and top box edges are the 25th to 75th percentiles, respectively, and whiskers indicate the range. Significant differences are indicated with asterisks (P < 0.01, two-tailed Mann-Whitney U test). Nine repeats from three independent biological replicates were performed. D, PhosTag western blot (WB) and WB analysis with anti-SUS antibodies. Total protein extract from 14-d-old whole roots of the wild type, OX, and Ri was assayed. Four biological replicates were performed.