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. 2020 Apr 3;183(2):733–749. doi: 10.1104/pp.19.01335

Figure 4.

Figure 4.

Glucosinolate accumulation remains elevated in the jazD phyB mutant. A, Simplified pathway for the biosynthesis of indole glucosinolates from central carbon metabolites erythrose 4-phosphate (E4P) and phosphoenolpyruvate (PEP). As a key intermediate in this pathway, Trp is both an essential amino acid for growth and a precursor for defensive glucosinolates. B and C, RT-qPCR measurements of relative transcript levels of Trp biosynthesis genes (ASA1, PAT1, IGPS, TSA1, and TSB2; B) and indole glucosinolate biosynthetic genes (CYP79B3, CYP83B1, CYP81F2, and IGMT1; C) in rosette leaves of the indicated genotypes. Expression levels were normalized to the reference gene PP2a. Data points show the means ± sd (n = 3 plants per genotype). D, Accumulation of indole glucosinolates in rosette leaves of wild-type (WT), jazD, phyB, and jazD phyB plants. Data points are means ± sd (n = 4 plants per genotype). Lowercase letters represent a significant difference at P < 0.05, determined by Tukey’s HSD mean-separation test. ASA1, Anthranilate synthase alpha subunit 1; PAT1, anthranilate phosphoribosyltransferase1; IGPS, indole-3-glycerol-phosphate synthase; TSA1, Trp synthase α chain1; TSB2, Trp synthase β chain2; IGMT1, indole glucosinolate O-methyltransferase1; I3M, indol-3-ylmethyl (glucobrassicin); 4OH-I3M, 4-hydroxyindol-3-ylmethyl (hydroxyglucobrassicin); 4MOI3M, 4-methoxyindol-3-ylmethyl (methoxyglucobrassicin); 1OH-I3M, 1-hydroxyindol-3-ylmethyl; 1MOI3M, 1-methoxyindol-3-ylmethyl (neoglucobrassicin).