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. 2020 Mar 31;183(2):671–685. doi: 10.1104/pp.20.00070

Figure 4.

Figure 4.

Met sulfoxidation decreases the DNA binding activity and transcription activity of NOR in vitro and in vivo. A, NOR sulfoxidation suppressed the DNA-binding activity of NOR, which were partially restored by E4 and SlMsrB2. B, Mimicking Met-138 sulfoxidation suppressed the DNA-binding activity of NOR. C, Mimicking Met-138 sulfoxidation significantly decreased NOR transactivation activities in vivo. D, Mimicking Met sulfoxidation in conserved M-H-E-Y-R motif significantly decreased the transactivation activities of SlNAC1, ONAC022, ANAC019, LcNAC1, and MaNAC1 in vivo. Each value represents the mean ± se of six biological replicates. Different letters above the bars indicated statistically significant differences between the samples (Student’s t test; P < 0.01). E, Subcellular localization of NOR and NOR-M138Q visualized by YFP analysis. Scale bars = 10 µm.