Figure 3.

Phylogenetic analysis of subfamily VIII R2R3-MYB TFs. A, ML phylogram of R2R3-MYB proteins of subfamily VIII in representative land plants. Clades within subfamily VIII are labeled and collapsed into triangles. Sequences from Zygnematophyceae were used as the outgroup. Ultrafast bootstrap values are associated with the internal branches. B, Schematic and alignment of VIII-A-, VIII-b/B-, and VIII-D-specific auxiliary motifs. Diagrams are not drawn to scale. C, Hypothetical evolutionary model of subfamily VIII TFs before the diversification of extant land plants. Circles represent inferred gene duplication events. Question marks indicate that there is conflict between motif analysis and topology.