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. 2020 Apr 2;183(2):656–670. doi: 10.1104/pp.20.00197

Figure 2.

Figure 2.

Expression patterns of vvi-miR171 and target genes during adventitious root formation and growth of cv Muscat Hamburg cuttings and the expression pattern of the vvi-MIR171 family in different tissues. A, Relative abundance of vvi-miR171 during adventitious root formation and growth of cuttings by stem-loop RT-qPCR. B, Cleavage sites of vvi-miR171 at complementary sequences of VvSCL15 and VvSCL27 mRNA as determined by 5′ RACE. The numbers of 5′ RACE clones corresponding to each site are indicated by arrows. The red boxes represent the GRAS domains of VvSCL15 and VvSCL27. Short black bars represent base matches, and black circles represent base mismatches. C, Expression patterns of VvSCL15 and VvSCL27 during adventitious root formation and growth of cuttings. The 3W to 10W indicate the time that the samples were collected beginning in the third week (14th d) after cuttings were prepared, and once a week until the 10th week. P, Phloem; R, root. D, Multiple sequence alignment of the vvi-miR171 family members. E, Expression pattern of vvi-MIR171 family in different tissues. Data are plotted as means ± sd, and error bars show the sd among three biological replicates (n = 3).