| Antibodies |
| Human polyclonal anti-Hu (ANNA-1) |
Gift of V. Lennon |
N/A |
| Rat monoclonal anti-MHC class II |
Millipore Sigma |
Cat#: MABF33; |
| Rat monoclonal anti-Somatostatin |
Merck |
Cat#: MAB354; RRID: AB_2255365 |
| Rabbit monoclonal anti-nNOS |
Abcam |
Cat#: ab76067, RRID: AB_2152469 |
| Rabbit polycolonal anti-ASC |
Adipogen |
Cat#: AG-25B-0006; RRID: AB_2490440 |
| Rabbit monoclonal anti-HA |
Cell Signaling Technology |
Cat#: 3724; RRID: AB_1549585 |
| Rabbit monoclonal S100 beta |
|
Cat#: ab52642 |
| Rat monoclonal anti-CSF1R (AFS98) |
Gift of M. Merad (MSSM) |
AFS98 |
| Anti-CD45.2 |
BD-Pharmingen |
Cat#: 560693 |
| Anti-CD45R |
BD-Pharmingen |
Cat#: 553088 |
| Anti-CD103 |
eBioscience |
Cat#: 11103185 |
| Anti-CD11b |
eBioscience |
Cat#: 47011282 |
| Anti-CD11c |
Affymetrix |
Cat#: 48011482 |
| Anti-F4/80 |
Affymetrix |
Cat#: 12480180 |
| Anti-Siglec F |
BD-Pharmingen |
Cat#: 562680 |
| Anti-CD3e |
Thermo Fisher Scientific |
Cat#: 45003180 |
| Anti-Ly6G |
BD-Pharmingen |
Cat#: 560601 |
| Anti-CD64 |
Biolegend |
Cat#: 139309 |
| Anti-MHC II |
Thermo Fisher Scientific |
Cat#: 17532380 |
| Aqua fixable dead cell stain |
Invitrogen |
Cat#: L34966
|
| Bacterial and Protozoan Strains |
|
S. enterica serovar Typhimurium (SL1344)
|
UC Davis, Andreas Bäumler |
Strain# SL1344; NCBI Taxonomy ID: 216597 |
|
S. enterica serovar Typhimurium (SL1344) ΔspiB
|
UC Davis, Andreas Bäumler |
N/A |
| Yersinia pseudotuberculosis |
NIH, Yasmine Belkaid |
|
|
Toxoplasma gondii (me49) |
UMass, Ricardo Gazzinelli |
me49 |
|
Trypanosoma cruzi (Y strain) |
UMass, Ricardo Gazzinelli |
N/A |
| Strongyloides venezuelensis |
Stanford, S. Galli |
N/A |
| Biological Samples |
| N/A |
N/A |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| Compound 21 |
Hello Bio |
Cat#: HB6124 |
| Tamoxifen |
Sigma |
Cat#: T5648 |
| Ampicillin sodium salt |
Sigma |
Cat#: A0166 |
| Neomycin trisulfate salt hydrate |
Sigma |
Cat#: N6386 |
| Vancomycin hydrochloride |
Sigma |
Cat#: V2002 |
| Metronidazole |
Sigma |
Cat#: M3761 |
|
|
|
| Salbutamol sulfate |
Selleck Chemicals |
Cat#: S2507 |
| Methylcellulose |
Sigma-Aldrich |
Cat#: 274429 |
| Carmine Red |
Sigma-Aldrich |
Cat#: C1002 |
| Spermine |
Sigma-Aldrich |
Cat#:85590 |
| DFMO |
Gift of P. Woster |
N/A |
| zVAD-FMK |
Selleck Chemicals |
Cat#: S7023 |
| TRIzol™ Reagent |
Thermo Fisher Scientific |
Cat#: 15596026 |
| Streptomycin sulfate |
Sigma-Aldrich |
Cat#: S9137 |
| HBSS, calcium, magnesium |
GIBCO |
Cat# 24020117 |
| HBSS, no calcium, no magnesium |
GIBCO |
Cat# 14170112 |
| Dulbecco’s Phosphate Buffered Saline |
Corning |
Cat# 21040CV |
| EDTA (0.5 M), pH 8.0 |
Ambion |
Cat# AM9260G |
| RPMI 1640 Medium |
GIBCO |
Cat# 11875119 |
| Collagenase VIII |
Sigma-Aldrich |
Cat# C2139 |
| DNAse I |
Roche |
Cat# 04536282001 |
| Isoflurane (IsoThesia) |
Henry Schein |
Cat# 029405 |
| DL-Dithiothreitol (DTT) |
Sigma-Aldrich |
Cat# D0632 |
| Triton X-100 |
Alfa Aesar |
Cat# A16046 |
| Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
Cat# D8418 |
| Tween 20 |
Promega |
Cat# H5151 |
| Heparin |
Sigma-Aldrich |
Cat# H3149 |
| Cuprolinic Blue |
Polysciences |
Cat#: 17052-100 |
| DNA extraction buffer |
Lucigen |
Cat#: QE09050 |
| RNAscope ® probe Nlrp6
|
ACDbio |
Cat#: 404561 |
| RNAscope ® probe Elavl4
|
ACDBio |
Cat#: 479581-C2 |
| PFHM-II |
Thermo Fisher Scientific |
Cat#: 12040077 |
| Dialysis Tubing |
Spectrum Labs |
Cat#: 132676 |
| Penicillin/streptomycin 100x |
Gibco |
Cat#: 15140-122 |
| Bioreactor Flask |
Wheation |
Cat#: WCL-1000-3 |
| 1M Tris-HCL pH 8 |
Invitrogen |
Cat#: 15568-025 |
| IgG Binding Buffer |
Thermo Fisher Scientific |
Cat#: 21011 |
| IgG Elution Buffer |
Thermo Fisher Scientific |
Cat#: 21004 |
| Protein G Sepharose |
GE Healthcare |
Cat#: 45-000-116 |
| Gravity Column |
Bio-Rad |
Cat#: 7371512 |
| 30K - Centrifugal Filters |
Amicon |
Cat#: UFC903024 |
| IgG from Rat Serum |
Sigma-Aldrich |
Cat#: I4131 |
|
|
|
| Critical Commercial Assays |
| Mouse Lipocalin-2/NGAL DuoSet ELISA |
R&D Systems |
Cat#: DY1857 |
| PicoPure RNA Isolation Kit |
Thermo Fisher Scientific |
Cat#: KIT0204 |
| RNAscope® Kit |
ACDBio |
Cat#: 320850 |
|
|
|
| Deposited Data |
| Data generated by RNA sequencing are deposited in the NCBI Gene Expression Omnibus (GEO) database. |
NCBI |
GSE140309 |
|
|
|
| Experimental Models: Organisms/Strains |
| Mouse: C57BL6/J |
Jackson Laboratory |
#000664 |
| Mouse: Lyz2Cre
|
Jackson Laboratory |
#004781 |
| Mouse: Rosa26tdTomato
|
Jackson Laboratory |
#007914 |
| Mouse: VGLUT2Cre
|
Jackson Laboratory |
#016963 |
| Mouse: 129S1/SvImJ |
Jackson Laboratory |
#002448 |
| Mouse: Ccr2−/−
|
Jackson Laboratory |
#004999 |
| Mouse: Casp1−/− Casp11−/−
|
Jackson Laboratory |
#016621 |
| Mouse: CBA/J |
Jackson Laboratory |
#000656 |
| Mouse: Rpl22HA
|
Jackson Laboratory |
#011029 |
| Mouse: Snap25Cre
|
Jackson Laboratory |
#023525 |
| Mouse: Adrb2flox
|
G. Karsenty |
N/A |
| Mouse: Arg1flox
|
Jackson Laboratory |
#008817 |
| Mouse: Cx3cr1GFP
|
|
|
| Mouse: NestinGFP
|
P. Frenette, G. Enikolopov |
N/A |
| Mouse: Casp11−/−
|
Jackson Laboratory |
#024698 |
| Mouse: R26-CAG-ASC-citrine |
Jackson Laboratory |
#030744 |
| Mouse: NSG |
Jackson Laboratory |
#005557 |
| Mouse: Phox2bcre
|
Jackson Laboratory |
# 016223 |
| #Mouse: Plp1CreERT
|
Jackson Laboratory |
# 005975 |
| Mouse: Rosa26DTA
|
Jackson Laboratory |
# 009669 |
| Mouse: R26-hM4Di/mCitrine |
Jackson Laboratory |
# 026219 |
| Mouse: Sox10CreERT2
|
B. Gulbransen, V. Pachnis |
N/A |
| Mouse: SNScre
|
R. Kuhner |
N/A |
| Mouse: Nlrp6flox
|
P. Rosenstiel |
N/A |
| Mouse: Casp11flox
|
KOMP |
N/A |
|
|
|
| Oligonucleotides |
|
Casp11 forward 5’-AGGCATATCTATAATCCCTTCACTG-3’ |
IDT |
N/A |
|
Casp11 reverse 5’-GAATATATCAAAGAGATGACAAGAGC- 3’ |
IDT |
N/A |
|
Arg1 forward 5’-CTCCAAGCCAAAGTCCTTAGAG-3’ |
IDT |
N/A |
|
Arg1 reverse 5’-AGGAGCTGTCATTAGGGACATC-3’ |
IDT |
N/A |
|
Ym1 forward 5’-AGACTTGCGTGACTATGAAGCATT-3’ |
IDT |
N/A |
|
Ym1 reverse 5’-GCAGGTCCAAACTTCCATCCTC-3’ |
IDT |
N/A |
|
Rpl32 forward 5’-ACAATGTCAAGGAGCTGGAG-3’ |
IDT |
N/A |
|
Rpl32 reverse 5’-TTGGGATTGGTGACTCTGATG-3’ |
IDT |
N/A |
|
Nlrp6 E1 forward 5’-TTGACTGTCAGCAAGAGTCC-3’ |
IDT |
N/A |
|
Nlrp6 E1 reverse 5’-GGTGATCCTTTCTGGGCTAAA-3’ |
IDT |
N/A |
| Nlrp6 E4 forward 5’-CAGACGCTGTGGACCTTGT-3’ |
IDT |
N/A |
| Nlrp6 E4 reverse 5’- ACGTGCTCGCGGTACTTCTT-3’ |
IDT |
N/A |
| Elavl4 forward 5’-GAT CAGGGATGCTAACCTGTATG-3’ |
IDT |
N/A |
| Elavl4 reverse 5’- GGTGATGATGCGACCGTATT -3’ |
IDT |
N/A |
| Recombinant DNA |
| AAV9-hSyn-eGFP-WPRE-bGH |
Addgene |
#105539-AAV9 |
| AAV9-hSyn-HI-eGFP-Cre-WPRE-SV40 |
Addgene |
#105540-AAV9 |
|
|
|
| Software and Algorithms |
| GraphPad Prism version 8 for MacOS |
Graphpad Software Inc. |
https://www.graphpad.com |
| RStudio |
RStudio® |
https://www.rstudio.com |
| DEseq2 |
Bioconstructor |
https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Imaris (v. 8.4) |
Bitplane |
|
| Fiji |
ImageJ |
https://imagej.net/Welcome |
| Microsoft Excel for MacOS |
Microsoft |
https://products.office.com/en-us/excel |
| Other |
| Salmonella Shigella Agar (SS Agar) |
BD |
Cat# 211597 |
| Luria’s Broth base (LB) |
Thermo-Fisher Scientific |
Cat# 12795027 |
| O.C.T. Compound (Tissue-Plus®) |
Fisher Healthcare |
Cat# 23-730-571 |
|
|
|
