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. 2020 Jun 4;15(6):e0233880. doi: 10.1371/journal.pone.0233880

Fig 4. The relative gene expression of the developmentally important related genes.

Fig 4

Real-time reverse-transcriptase PCR gene expression for the three pluripotency genes (POU5F1, NANOG, and SOX2), SUV39H1/H2, and DNMT3A/B in the blastocysts derived from the IVF, non-treated SCNT (SCNT-CRL) and chaetocin, TSA, chaetocin + TSA, and siSUV39H1/H2 treated SCNT. Fold-change values were calculated from three biological replications following normalization to β-ACTIN. Each biological replication contains six blastocyst in each group. Values are presented as mean ± standard error of mean. Different letters indicate significant differences between the groups at P< 0.05.