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(a) The 1H-15N HSQC spectra of 15N MDM2 in complex with unlabeled Peptide 1 (red) in a 1:1 ratio. (b) The 1H-15N HSQC spectra of 15N MDM2 in complex with unlabeled p53 (blue) in a 1:1 ratio. To the same mixture, we added the Peptide 1 in a 1:1 ratio and obtained the spectrum as shown in (c). Then the spectra obtained in (b) and (c) is overlapped to the spectra indicated in (a) and the resulting overlapped spectra are shown in (d).
