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. 2020 Jun 4;84:104390. doi: 10.1016/j.meegid.2020.104390

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Fig. 5 — Genetic diversity at the target region of the Zhu_2020_CDC-China_Set II assay. Three Colombian genomes, hCoV/Colombia/Bogota/78390/2020, hCoV/Colombia/ Valle_del_Cauca/81279/2020 and hCoV/Colombia/Valle_del_Cauca/81251/2020 have the same pattern of substitutions (GGG to AAC) in primer Zhu_2020_CDC-China_Set II Fw (N) in the first three nucleotides at the 5′ end (highlighted in red). Furthermore, the hCoV/Colombia/Quindio/79911/2020 and hCoV/Colombia/Quindio/80663/2020 genomes displayed a substitution (G to T) at position 28,899 where this oligonucleotide hybridizes (highlighted in red). Genomic positions were estimated according to the SARS-CoV-2 reference genome available at GenBank (NC_045512.2). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)