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Yield comparison and size distribution for gel electrophoresis and ITP-based methods. (a) RNA size distribution was determined using Agilent Bioanalyzer 2100. The solid lines represent three replicates from ITP size selection method and the dotted lines denote the three replicates from gel electrophoresis method. (b) Concentration of extracted RNA in the desired size range of 17 – 35 nt was quantified. Gray lines indicate the mean values of the three replicates from each method.
