Figure 5.

Comparison between high-throughput sequencing results of RNA fragments recovered by ITP and gel electrophoresis methods. (a) Reads mapping to the coding region, 3’ and 5’ UTR were counted and plotted for both methods (three replicates each). (b) The mean number of reads per transcript across the three replicates of each method was calculated and compared. See Figure S4 for replicate-level comparisons. (c) For each transcript, the mean and standard deviation of log₂ read count were calculated across the replicates. A cubic spline was fitted and plotted for each method. See Figure S11 for the individual data points showing each transcript.