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. Author manuscript; available in PMC: 2020 Jun 20.
Published in final edited form as: Leukemia. 2019 Dec 20;34(6):1588–1598. doi: 10.1038/s41375-019-0682-7

Figure 1. GS-5829 induces apoptosis and suppresses proliferation of CLL cells.

Figure 1.

(A) Primary CLL cells co-cultured with NLC were treated for 120 hours with increasing concentrations of GS-5829 that are indicated on the horizontal axis. The mean fraction of viable CLL cells with 95% CI is depicted for each experimental condition (N=7). (B) The BET inhibitors GS-5829 and JQ1 dose-dependently inhibit the metabolism/proliferation of MEC-1 cells, as measured in XTT assays. The tested concentration range is shown on the horizontal axis. Displayed is the mean (± SD) absorbance of the colored formazan product, based on triplicate measurements that were normalized to average control values. (C) GS-5829 significantly inhibits the proliferation of MEC-1 cells, based on cell counts after 96 hours of exposure to different GS-5829 concentrations (D). Inhibition of proliferation was not due to induction of MEC-1 cell death, given that cell viability under these conditions remained high and not significantly changed when compared to control cells. Displayed are the mean with 95% CI from 3 independent experiments.