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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Cancer Res. 2020 Mar 10;80(11):2298–2310. doi: 10.1158/0008-5472.CAN-19-3133

Figure 3. PD-1 glycosylation is critical for maintaining its stability and membrane expression.

Figure 3.

(A) Schematic diagram of various PD-1 NQ mutants. The numbers indicate the amino acid positions on PD-1. (B) Flow cytometric analysis of cell surface PD-1 WT or the indicated NQ mutants over-expressed in Jurkat T cells. (C) Protein half-life of PD-1 WT or the indicated NQ mutants over-expressed in Jurkat T cells. Cells were treated with CHX for the indicated time, and PD-1 levels were examined by immunoblotting. (D) Protein half-life of PD-1 WT or 4NQ overexpressed in 293T cells. Cells were treated with CHX for the indicated time and PD-1 levels were examined by immunoblotting. (E) Ubiquitination of PD-1 WT or 4NQ purified from 293T cells treated with or without MG132. (F) Immunoblot of PD-1 4NQ in 293T cells treated with CHX for the indicated time in the presence or absence of MG132.