In vitro and in vivo γH2AX analysis after combined radiation and AZD4547 treatment in NCI-H226 and NCI-H1581 cell lines. Representative immunofluorescence γH2AX foci images and foci quantification for (A) NCI-H226 cells grown in vitro, (B) NCI-H226 cells grown in vivo and (C) NCI-H1581 cells grown in vivo. In vitro NCI-H226 cells were treated with DMSO, AZD4547 (0.1 μM), radiation (3 Gy) or combined AZD4547 (0.1 μM) and radiation (3 Gy) and analyzed at designated time points. NCI-H1581 and NCI-H226 xenografts were treated with DMSO, AZD4547 (6.25 or 12.5 mg/kg), radiation (2 Gy single dose) or combined AZD4547 (6.25 or 12.5 mg/kg) and radiation (2 Gy single dose). Columns, mean; bars, SEM (n=3).