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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2020 Mar 27;19(6):1266–1278. doi: 10.1158/1535-7163.MCT-19-0174

Figure 2. BIGH3 knockdown increased OSB differentiation.

Figure 2.

A) BIGH3 mRNA levels in Bo-786 cells with BIGH3 knockdown (Bo-786/BG#3sh and Bo-786/BG#4sh) were confirmed by real-time RT-PCR. B) ALP mRNA and protein levels were determined by real-time RT-PCR and by ALP activity assay. C) OSC mRNA levels were determined by real-time RT-PCR and OSC protein levels were determined using OSC ELISA kit. D) Calcium deposits were determined by calcium assay. *: P < 0.05; **: P < 0.01 as compared to its control.