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. 2020 Mar 3;22(6):1005–1014. doi: 10.1038/s41436-020-0766-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Receiver operating characteristic (ROC) curves and area under the curve (AUC) comparing in silico methods for predicting splice disruption in overlapping set of experimentally validated variants scored by all measures (136 non–splice disrupting, 70 splice disrupting). HSF human splicing finder, MES MaxEntScan (Alamut), NN NNSplice (Alamut), SSF SpliceSiteFinder (Alamut). Ala23 = number of Alamut tools exceeding specified thresholds.