Skip to main content
. 2020 May 27;133(10):jcs239723. doi: 10.1242/jcs.239723

Fig. 5.

Fig. 5.

Multipolar mitosis due to CSAG1 depletion is affected by p53 expression. (A) Multipolar mitosis (in %) in RPE1 cells that had been depleted of p53 with shRNA and transfected with CSAG1 siRNA or negative (NC control siRNA). A total of 150 cells was analyzed from two independent experiments. A Kruskal–Wallis test was used for statistical analysis, error bars represent +s.e.m. (B) The number of multipolar mitotic cells (in %) were determined in HCT116 parental or p53 KO cells after treatment with NC or CSAG1 siRNA. A total of >300 cells was analyzed for mitotic defects from three independent experiments. Error bars represent +s.d. Two way ANOVA with Sidak's multiple comparisons test was used for statistical analysis. (C) Elapsed time from nuclear envelope breakdown (NEBD) to anaphase was determined for cells shown in A that did not show mitotic defects. A Kruskal-Wallis test was used for statistical analysis. A total of >200 cells from three independent experiments was analyzed. CSAG1 depletion caused pronounced multipolar mitosis in p53 KO cells, and increased mitotic duration in both HCT116 parental and p53 KO cells.