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. 2020 May 27;133(10):jcs240416. doi: 10.1242/jcs.240416

Fig. 2.

Fig. 2.

Identification of functional, evolutionarily conserved residues in the 231–282 LBD. (A) Diagram of PRR14 depicting the minimal 231–282 LBD. The LAVVL HP1/heterochromatin-binding motif (52–56) is indicated in red. (B) Protein sequence alignment of the human 231–282 PRR14 LBD with mouse, Xenopus and gecko orthologs identified conserved residues: charged (gray) and hydrophobic (orange). Amino acid sequence identity (*) and similarity (.) are indicated. (C) Representative confocal images of HeLa cells transfected with GFP-tagged PRR14 231–282 LBD containing the indicated amino acid substitutions. (D) Box plot demonstrating the proportion of the indicated PRR14-GFP proteins at the nuclear lamina compared to the total nuclear signal, calculated using Lamin A/C signal as a mask. Boxes indicate the interquartile range with the median represented by a horizontal bar. Whiskers are drawn using the Tukey method and + indicates the mean value. n=20 cells per condition. ****P<0.0001; ns, not significant (one-way ANOVA with Dunn's multiple comparison test). Scale bars: 5 µm.