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. 2020 May 29;9(5):bio045260. doi: 10.1242/bio.045260

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — The effect of HSDs on climbing capacity and mortality in aging flies. (A) An image of HSD flies drinking water. (B) Time to fatigue of 1-week-old flies. (C) Time to fatigue of 3-week-old flies. (D) Time to fatigue of 5-week-old flies. (E) Time to fatigue in w1118 flies. (F) Time to fatigue in 2%-SD flies. (G) Time to fatigue in 4%-SD flies. (H) Time to fatigue in 8%-SD flies. (I) The climbing index changes with aging. (J) The climbing index in HSD flies. (K) The curves of survival and the average lifespan. Using a non-parametric followed by a log-rank test to analyze ‘survival’ and ‘time to fatigue’. The one-way analysis of variance (ANOVA) with least significant difference (LSD) tests were used to identify differences among the ‘w1118’, ‘w1118+2%salt’, ‘w1118+4%salt’ and ‘w1118+8%salt’ flies. Data are represented as means±s.e.m. *P<0.05; **P<0.01; ***P<0.001.