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. 2020 May 29;9(5):bio045260. doi: 10.1242/bio.045260

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 7. — The effect of dFOXO knockdown on salt tolerance of HSD flies. (A) Time to fatigue of 1-week-old flies. (B) Time to fatigue of 3-week-old flies. (C) Time to fatigue of 5-week-old flies. (D) The climbing index changes with aging in salt-overexpression flies. (E) The climbing index. (F) The curves of survival. (G) The average lifespan. (H) The salt expression. (I) The dFOXO expression. (J) The SOD activity level. (K) The MDA level. Using a non-parametric followed by a log-rank test for analyze survival and time to fatigue. One-way ANOVA with LSD tests were used to identify differences among the ‘dFOXO-control’, ‘dFOXO-knockdown’, and ‘dFOXO-knockdown+HSD’ flies. Data are represented as means±s.e.m. *P<0.05; **P<0.01.