Fig. 5.
Time-dependent maturation of the neurophysiological properties of human cells in FTDP-17: preserved HCN-channel function during extended neurogenesis. (A) Voltage drop and sag ratio for a hyperpolarizing current pulse of −100 pA in control (non-demented) and FTDP-17 groups at 100 and 150 DIV (Vdrop, n=17 in control, n=6 in FTDP-17 at 100 DIV and n=17, n=32 at 150 DIV; sag ratio, n=17 in control, n=6 in FTDP-17 at 100 DIV and n=16, n=32 at 150 DIV). (B) Left, representative western blot for HCN1 in iPSC-derived neurons in two control lines (C1 and C2) and two FTDP-17 lines (i and ii) at 150-165 DIV. β-actin was used as a loading control. Right, HCN1 protein level (relative for β-actin) in control and FTDP-17 samples (n=4 samples in control and n=5 in FTDP-17; three independent experiments performed). Lines depict median values. Data are mean±s.e.m. unless indicated otherwise. *P<0.05; ***P<0.001 (150 DIV versus 100 DIV for control, ANOVA with Bonferroni post-hoc test); #P<0.05; ###P<0.001 (150 DIV versus 100 DIV for FTDP-17).