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. 2020 Jun 4;10:9146. doi: 10.1038/s41598-020-66156-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of inflammatory mediators after infection with Blastocystis.(a,b) Quantification of Interleukin-6 (IL-6) (a) and Lipocalin-2 (b) in colonic tissues of control (n = 5) and infected rats (n = 11) at D31. Values were expressed as pg/mg protein. (c) Colonic expression of GATA-3 in control (n = 6) and infected rats (n = 11) at D31. (d) FITC-Dextran 4 kDa concentration in serum of control (n = 6) and infected (n = 11) rats at D21. Colonic expression of ZO-1 (e) and occludin (f) in control (n = 6) and infected rats (n = 11) at D31. (g) Serine protease activity (U/mg feces) in supernatants from feces of control (n = 6) and infected rats (n = 12) at D31. (h) Correlation between serine protease activity and AUC of colonic response. Statistical analysis: Values were expressed as relative expression compared to GAPDH expression. Statistical analysis: a and b, Mann-Whitney test: c, d, e, f, g and h t-test; Spearman-test; *p < 0.05 **p < 0.01; ***p < 0.001; three independent experiments were performed.