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. 2020 Jun 4;10:9144. doi: 10.1038/s41598-020-66051-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vulcanoplot of peptides derived from Mal d 1 isoforms showing contrasting contents in apples of the cultivars Santana and Golden Delicious. The peptides with a positive log10 Fold Change (FC) were mainly present in Golden Delicious (GD), the peptides with a negative FC were mainly found in Santana. The peptides that are specific for single Mal d 1 isoforms or small groups are marked in red, more general peptides are black. Santana: Group of Mal d 1.03 proteins (1), CBL94177.1_Mal d 1.08 (2), group of Mal d 1.06 proteins (3), CBL94138.1_Mal d 1.10 (4), XP_008351173.1_Mal d 1-like protein (5) Golden Delicious: AAS00045.1_Mal d 1-like protein (6) and AAX20977.1_Mal d 1.06B or AAS00045.1_Mal d 1- like protein (7) (UniprotKB). Significance difference was calculated at significance level of 3% (p value ≤ 0.03). The image was generated with RStudio 3.6.0 (https://rstudio.com).