Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 4;11:2817. doi: 10.1038/s41467-020-16309-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schematic of the conditionally activated endogenous alleles of KRAS^G12D and AGO2 used in the study to generate the AGO2^fl/fl;KRAS^G12D;p48Cre experimental mice. b Representative images of H&E and AGO2 IHC analysis of pancreata obtained from AGO2^+/+;p48Cre and AGO2^fl/fl;p48Cre genotypes. Orange and black arrows indicate AGO2 expression in acinar cells and islets of Langerhans, respectively. Scale bar, 100 µm. c Representative H&E and IHC analysis for AGO2 in pancreata obtained from 12-week old mice from the AGO2^+/+;KRAS^G12D;p48Cre and AGO2^fl/fl;KRAS^G12D;p48Cre genotypes. Orange and black arrows indicate AGO2 staining in the PanIN and stromal regions, respectively. Scale bar, 100 µm. d Scatter plot showing the weight of pancreata obtained from 10 AGO2^+/+;KRAS^G12D;p48Cre, 17 AGO2^fl/+;KRAS^G12D;p48Cre, and 14 AGO2^fl/fl;KRAS^G12D;p48Cre mice aged over 400 days. Two sided t-test was performed to determine the P value and error bars are mean values +/− SEM. e Histogram showing average number of early and late PanIN lesions observed in 11 mice each of AGO2^+/+;KRAS^G12D;p48Cre, AGO2^fl/+;KRAS^G12D;p48Cre, and AGO2^fl/fl;KRAS^G12D;p48Cre genotypes at 400 days. The number of early/ late PanINs and PDAC within pancreatic sections from each animal were counted as a percentage. For AGO2^fl/fl;KRAS^G12D;p48Cre mice, only lesions that do not express AGO2 have been included. f Kaplan–Meier curve for tumor-free survival of AGO2^+/+;KRAS^G12D;p48Cre, AGO2^fl/+;KRAS^G12D;p48Cre, and AGO2^fl/fl; KRAS^G12D;p48Cre mice aged over 500 days. g Chart showing PDAC (within the pancreas), the different metastatic lesions, and abnormal pathologies (black boxes) observed in each mouse of the indicated genotypes aged over 500 days. Gray boxes in the AGO2^fl/fl;KRAS^G12D;p48Cre group indicate abnormal pathology observed at the indicated site and are addressed in further detail in Supplementary Fig. 4. The number of mice indicated in this figure represent biologically independent individuals.