Fig. 1.

Workflow for the recovery of levansucrase containing supernatants and for the subsequent levan production at different pH (chapter 3.2). The pH values in brackets indicate the determined final production pH after mixing of the respectively used buffers. Three independently grown cell cultures (300 mL; OD 2.2, 2.58, 2.84) were prepared and handled as depicted. The obtained results about amounts and sizes of the recovered levans as well as contained volumetric levansucrase activities (in total 15 samples per cell culture: 5 release pH × 3 production pH) were finally compared among each other (Fig. 3 and Supplementary File 1)