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. 2020 May 28;8:414. doi: 10.3389/fchem.2020.00414

Table 1.

Binding constants (K, M−1)a obtained from fluorescence titration.

Anionb Oxalate Citrate Malonate Acetate Pi PPi F
K11 K21
1 (7.1 ± 0.5) × 104 (1.1 ± 0.1) × 104 (4.2 ± 0.8) × 103 N.D.c (1.6 ± 0.1) × 105 (3.0 ± 0.1) × 104 N.D.c >106
2 (1.1 ± 0.1) × 105 N.D.c (8.2 ± 1.0) × 104 (1.3 ± 0.2) × 105 <103 > 108 N.D.c >106
a

Binding constants were calculated using the change in fluorescence titrations at 25°C.

b

Small response was observed for the addition of Cl, Br, and I. Thus, the K values could not be calculated.

c

The K value could not be calculated due to sigmoidal responses.