Figure 3.

Suppressing adipocyte inflammation causes further insulin resistance upon obese pre-conditioning. (A) Mice were fed HFD only (no dox) for 30 days and then switched to HFD-dox for another 10 weeks. A slightly reduced body weight is observed in RID^ad mice. N = 9–12/group. (B) One-week acute induction of RIDα/β results in comparable glucose tolerance tests. N = 9–12/group. (C-D) Prolonged HFD-dox exposure (8 weeks) impairs glucose and insulin tolerance in RID^ad mice. N = 9–12/group. (E) Elevated serum insulin levels in RID^ad mice. N = 5–7/group. (F) H&E staining of liver, BAT, gWAT, and sWAT. Scale bar, 100 μm.