Fig. 5.

Effect of lutein on the expression of redox markers in ARPE-19 cells upon glucose induction. Protein expression of SOD2 (a), catalase (b) and HO-1 (c), and GSH levels (d) in cellular extract of ARPE-19 pre-treated with lutein (1 μM) for 3 h followed by glucose (25 mM) for 24 h was detected by western blotting as described in materials and methods. Values are mean ± SD (n = 3); Bars with different letter indicate significant difference (p < 0.05) between the group. d GSH levels were assessed using GSH assay kit after pre-treatment with lutein (1 μM) followed by glucose (25 mM) treatment. Values are mean ± SD (n = 3); Bars with different letter indicate significant difference (p < 0.05) between the group