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. 2020 May 28;11(2):289–309. doi: 10.1007/s13167-020-00209-y

Table 2.

The proteins corresponding to 116 EIF4A3-biding mRNAs and EIF4A3 were identified by SILAC-based quantitative proteomics in ovarian cancer cells treated with (H) and without (L) ivermectin

Protein IDs Protein names Gene names Unique peptides Sequence coverage [%] Mol. weight [kDa] Sequence length Q-value Score Intensity H Intensity L Ratio H/L Ratio H/L variability [%]
A0A0S2Z4C6 Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform PPP3CA 6 22.60 58.69 521 0 106 450,040,000 2,321,300,000 0.17 59.00
A0A024R7B0 Ubiquitin-like protein 5 UBL5 1 12.30 8.55 73 0.0014951 3 33,400,000 156,360,000 0.20 4.63
A0A024R9A9 Ubiquitin-conjugating enzyme E2 T UBE2T 3 18.30 22.52 197 0 7 54,580,000 216,150,000 0.22 6.66
A0A494C101 Pyruvate carboxylase; pyruvate carboxylase, mitochondrial PC 2 5.20 53.49 483 0.00078309 3 3,454,600 19,685,000 0.28 NaN
A0A1W2PNM1 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial HADH 4 21.30 29.10 267 0 11 85,389,000 332,200,000 0.30 14.69
Q496I0 Cytochrome c oxidase subunit 7A2, mitochondrial COX7A2 2 27.70 9.43 83 0 10 223,550,000 687,660,000 0.32 44.38
Q149N6 Dedicator of cytokinesis protein 4 DOCK4 3 1.70 225.28 1966 0 4 2,402,200 12,153,000 0.33 NaN
Q15036 Sorting nexin-17 SNX17 3 9.30 49.75 440 0 9 2,545,600 64,295,000 0.33 NaN
J3KN67 Tropomyosin alpha-3 chain TPM3 0 36.50 33.22 285 0.0010344 3 44,208,000 225,430,000 0.34 7.74
Q8WZ82 Ovarian cancer-associated gene 2 protein OVCA2 5 40.10 24.42 227 0 8 22,175,000 92,294,000 0.34 32.53
G3V0I5 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial NDUFV1 4 12.30 50.05 457 0 9 25,550,000 98,424,000 0.34 50.52
J3QLR8 28S ribosomal protein S23, mitochondrial MRPS23 3 19.70 17.52 152 0 8 59,957,000 127,510,000 0.35 11.23
J3KSI8 28S ribosomal protein S7, mitochondrial MRPS7 3 22.40 18.27 156 0 9 56,198,000 98,108,000 0.35 9.42
B4DP80 NAD(P)H-hydrate epimerase APOA1BP 6 30.90 33.63 307 0 47 81,014,000 347,980,000 0.37 20.29
C9JFE4 COP9 signalosome complex subunit 1 GPS1 5 13.40 53.37 471 0 25 189,930,000 447,320,000 0.38 30.48
Q9Y3B7 39S ribosomal protein L11, mitochondrial MRPL11 2 18.20 20.66 192 0 5 17,279,000 5,213,500 0.38 NaN
Q9Y333 U6 snRNA-associated Sm-like protein LSm2 LSM2 1 20.00 10.83 95 0 23 188,570,000 423,780,000 0.38 10.47
P63261 Actin ACTG1 1 94.10 41.79 375 0 212 3,863,900,000 16,738,000,000 0.38 20.93
Q07954 Prolow-density lipoprotein receptor-related protein 1 LRP1 3 1.20 504.60 4544 0 5 3,971,000 52,428,000 0.38 57.69
Q68E01 Integrator complex subunit 3 INTS3 6 7.70 118.07 1043 0 11 32,873,000 113,610,000 0.38 24.03
K7EKI4 39S ribosomal protein L4, mitochondrial MRPL4 2 27.60 14.06 127 0 9 41,947,000 121,900,000 0.39 45.85
V9GZ56 U6 snRNA-associated Sm-like protein LSm4 LSM4 2 7.10 25.73 238 0 4 89,988,000 217,330,000 0.39 7.01
A0A0S2Z4T1 DNA replication licensing factor MCM3 MCM3 22 33.20 90.98 808 0 187 1,032,600,000 3,186,200,000 0.40 25.19
A0A0S2Z3L0 Electron transfer flavoprotein subunit alpha, mitochondrial ETFA 9 37.80 35.08 333 0 44 623,280,000 1,381,500,000 0.40 22.23
Q8NFH5 Nucleoporin NUP53 NUP35 7 31.60 34.81 326 0 81 206,630,000 398,870,000 0.40 25.90
Q5T7C4 High mobility group protein B1 HMGB1 7 54.40 18.31 158 0 98 2,916,000,000 11,413,000,000 0.40 30.66
A0A024R8M4 Phosphoribosyl pyrophosphate synthase-associated protein 1 PRPSAP1 5 25.80 39.39 356 0 20 88,227,000 200,670,000 0.41 23.09
G3V2D5 Zinc finger protein 36, C3H1 type-like 1 ZFP36L1 3 20.50 19.53 176 0 18 6,238,500 15,019,000 0.41 45.33
A6NMQ3 Alpha-endosulfine ENSA 1 20.00 15.63 140 0 5 80,231,000 206,330,000 0.41 30.68
A0A0J9YYL3 Poly(U)-binding-splicing factor PUF60 PUF60 17 42.40 54.63 505 0 135 468,910,000 1,280,500,000 0.42 24.83
A0A481SVJ4 Matrix-remodeling-associated protein 7 MXRA7 2 20.90 15.43 139 0.0026961 2 4,184,200 24,167,000 0.42 NaN
J3KTF8 Rho GDP-dissociation inhibitor 1 ARHGDIA 7 44.00 21.52 193 0 56 1,439,500,000 3,590,300,000 0.42 56.40
P49736 DNA replication licensing factor MCM2 MCM2 20 27.50 91.27 808 0 90 937,140,000 2,544,400,000 0.43 37.42
K7EJH0 Kinetochore protein Spc24 SPC24 2 20.70 15.97 140 0 7 91,278,000 196,000,000 0.43 6.67
Q9BRA2 Thioredoxin domain-containing protein 17 TXNDC17 4 38.20 13.94 123 0 38 745,240,000 1,838,200,000 0.43 13.73
P15531 Nucleoside diphosphate kinase A NME1 1 69.70 17.15 152 0 20 312,380,000 966,090,000 0.43 5.96
D3DVA5 Rho guanine nucleotide exchange factor 2 ARHGEF2 6 7.60 116.00 1030 0 16 26,979,000 80,246,000 0.44 68.74
B7ZM10 Exportin-6 XPO6 3 3.20 127.42 1111 0 7 7,924,200 14,822,000 0.44 28.28
G8JLD3 ELKS/Rab6-interacting/CAST family member 1 ERC1 12 14.10 124.82 1086 0 44 179,630,000 428,430,000 0.44 47.40
B2R7W3 Breast carcinoma amplified sequence 2 BCAS2 3 16.90 26.13 225 0 15 38,190,000 177,190,000 0.44 7.05
C9JJ19 28S ribosomal protein S34, mitochondrial MRPS34 5 25.30 26.33 225 0 10 69,862,000 158,490,000 0.44 15.88
A0A0S2Z4Q4 Hepatocyte growth factor-regulated tyrosine kinase substrate HGS 4 6.40 74.85 661 0 10 52,165,000 121,060,000 0.44 18.90
Q53Y51 D-dopachrome decarboxylase DDT 4 39.80 12.71 118 0 36 259,770,000 672,160,000 0.45 4.37
A0A024R8U9 Pyrroline-5-carboxylate reductase 1, mitochondrial PYCR1 7 33.90 33.39 319 0 20 72,360,000 265,100,000 0.45 16.22
Q6FHQ0 Histone-binding protein RBBP7 RBBP7 9 41.90 47.82 425 0 160 2,134,000,000 4,792,100,000 0.45 24.67
P29144 Tripeptidyl-peptidase 2 TPP2 23 24.90 138.35 1249 0 173 523,060,000 1,368,300,000 0.45 19.08
Q9UP83 Conserved oligomeric Golgi complex subunit 5 COG5 3 5.00 92.74 839 0 5 14,333,000 32,372,000 0.46 25.35
E9PID8 Cleavage stimulation factor subunit 2 CSTF2 3 19.20 46.67 438 0 39 128,240,000 239,460,000 0.46 57.11
A0A024R496 Calcium-binding protein 39 CAB39 4 12.90 39.87 341 0 24 198,430,000 418,650,000 0.46 24.48
Q6IAP9 U4/U6 small nuclear ribonucleoprotein Prp4 PRPF4 13 32.40 58.31 521 0 42 168,190,000 621,480,000 0.46 8.21
A0A024RB32 Prostaglandin E synthase 3 PTGES3 8 51.20 18.70 160 0 110 2,019,900,000 5,057,500,000 0.46 20.42
E9PMG1 RalBP1-associated Eps domain-containing protein 1 REPS1 2 3.80 80.44 737 0 8 13,894,000 28,102,000 0.46 19.89
P28066 Proteasome subunit alpha type-5 PSMA5 8 49.40 26.43 241 0 107 1,677,200,000 4,011,200,000 0.46 17.27
I3L2G3 Ketosamine-3-kinase FN3KRP 2 25.20 14.06 127 0 7 16,246,000 54,477,000 0.46 NaN
A0A0S2Z4Z0 RNA-binding protein 14 RBM14 13 23.80 69.49 669 0 83 606,470,000 1,445,500,000 0.46 15.69
A8K651 Complement component 1 Q subcomponent-binding protein, mitochondrial C1QBP 6 31.60 31.38 282 0 109 3,184,200,000 7,587,100,000 0.47 34.39
O60506 Heterogeneous nuclear ribonucleoprotein Q SYNCRIP 15 44.30 69.60 623 0 121 3,657,000,000 8,092,200,000 0.47 28.95
P42345 Serine/threonine-protein kinase mTOR MTOR 9 4.70 288.89 2549 0 14 52,206,000 144,780,000 0.47 6.97
A8K878 Mesencephalic astrocyte-derived neurotrophic factor MANF 8 40.50 21.14 185 0 38 604,460,000 1,612,900,000 0.47 14.42
A0MNN4 Shwachman-Bodian-Diamond syndrome isoform 1 SMU1 10 27.70 57.54 513 0 60 321,320,000 679,840,000 0.47 28.26
A0A024R8B1 TBC1 domain family member 13 TBC1D13 3 13.10 32.17 275 0 4 20,484,000 74,249,000 0.47 4.26
Q9UHR4 Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1 BAIAP2L1 2 6.50 56.88 511 0 6 21,391,000 41,855,000 0.47 9.59
Q5SRT3 Chloride intracellular channel protein 1 CLIC1 15 83.00 26.92 241 0 299 12,945,000,000 29,241,000,000 0.47 36.26
A0A0S2Z5I7 Ribosome maturation protein SBDS SBDS 8 32.00 28.76 250 0 16 344,540,000 808,860,000 0.48 11.35
Q13505 Metaxin-1 MTX1 5 15.50 51.46 466 0 10 45,737,000 109,770,000 0.49 42.70
J3KS15 Peptidyl-tRNA hydrolase ICT1, mitochondrial ICT1 1 7.30 21.91 192 0.0081075 2 29,017,000 45,629,000 0.50 21.01
Q53HN4 DNA fragmentation factor subunit alpha DFFA 1 19.30 36.59 331 0 31 272,390,000 539,710,000 0.50 12.11
P38919 Eukaryotic initiation factor 4A-III EIF4A3 14 38.20 46.87 411 0 77 1,570,400,000 3,533,500,000 0.50 16.42
B4DY09 Interleukin enhancer-binding factor 2 ILF2 15 58.80 38.91 352 0 96 2,641,700,000 6,060,100,000 0.50 41.63
E9PF19 Transducin beta-like protein 2 TBL2 5 19.20 35.38 318 0 28 109,400,000 213,970,000 0.50 25.21
A0A087WXS7 ATPase ASNA1 ASNA1 8 33.50 37.12 331 0 63 850,190,000 1,754,700,000 0.51 20.08
O43324 Eukaryotic translation elongation factor 1 epsilon-1 EEF1E1 6 35.10 19.81 174 0 29 662,460,000 1,235,700,000 0.51 34.40
Q15717 ELAV-like protein 1 ELAVL1 10 41.70 36.09 326 0 92 2,093,100,000 4,319,900,000 0.51 13.01
Q9UMS4 Pre-mRNA-processing factor 19 PRPF19 10 35.30 55.18 504 0 127 897,150,000 2,364,000,000 0.52 19.98
P14324 Farnesyl pyrophosphate synthase FDPS 3 16.20 48.28 419 0 26 1,029,900,000 2,078,100,000 0.52 12.36
P28070 Proteasome subunit beta type-4 PSMB4 5 28.40 29.20 264 0 25 512,090,000 1,000,200,000 0.52 9.30
Q0VGA5 SARS protein SARS 12 30.30 58.41 511 0 149 1,216,000,000 2,647,300,000 0.53 20.86
A0A024RCX8 Peptidylprolyl cis-trans isomerase-like 1 PPIL1 4 27.10 18.24 166 0 10 122,810,000 365,070,000 0.53 29.74
Q9H8H0 Nucleolar protein 11 NOL11 4 9.00 81.12 719 0 5 22,443,000 80,063,000 0.54 13.01
E7EX90 Dynactin subunit 1 DCTN1 24 27.00 139.09 1256 0 131 823,150,000 1,803,200,000 0.54 26.60
Q05D78 Double-strand break repair protein MRE11A MRE11A 3 6.00 59.72 517 0.00078125 3 24,064,000 56,350,000 0.54 14.57
H7C440 DIS3-like exonuclease 2 DIS3L2 3 6.50 43.54 383 0 4 13,696,000 21,117,000 0.54 31.74
Q9Y3U8 60S ribosomal protein L36 RPL36 5 30.50 12.25 105 0 34 544,540,000 770,200,000 0.55 42.00
Q9UJZ1 Stomatin-like protein 2, mitochondrial STOML2 9 34.80 38.53 356 0 119 866,230,000 1,683,300,000 0.55 16.00
Q567R6 Single-stranded DNA-binding protein, mitochondrial SSBP1 5 39.20 17.36 148 0 60 561,820,000 1,076,800,000 0.55 28.12
Q15084 Protein disulfide-isomerase A6 PDIA6 18 44.80 48.12 440 0 323 12,005,000,000 20,233,000,000 0.56 25.50
Q15645 Pachytene checkpoint protein 2 homolog TRIP13 7 19.00 48.55 432 0 27 276,670,000 492,930,000 0.56 13.39
A0A024R6K8 Epididymis secretory sperm binding protein WARS 14 41.00 53.17 471 0 80 1,768,700,000 2,802,900,000 0.56 22.11
Q9NPD3 Exosome complex component RRP41 EXOSC4 4 19.60 26.38 245 0 25 239,980,000 490,570,000 0.57 6.00
Q9UHB9 Signal recognition particle subunit SRP68 SRP68 13 27.40 70.73 627 0 68 343,910,000 687,640,000 0.57 56.97
F5H0P4 Porphobilinogen deaminase HMBS 6 28.10 25.33 228 0 9 38,322,000 129,490,000 0.57 80.17
Q96SB4 SRSF protein kinase 1 SRPK1 7 14.80 74.32 655 0 49 123,510,000 199,050,000 0.57 23.79
Q9Y6W5 Wiskott-Aldrich syndrome protein family member 2 WASF2 4 11.00 54.28 498 0 22 69,082,000 151,250,000 0.57 28.41
A0A024R8S5 Protein disulfide-isomerase P4HB 28 60.20 57.12 508 0 300 16,556,000,000 27,987,000,000 0.57 20.59
A0A2X0SF71 Rho GTPase-activating protein 17 ARHGAP17 8 17.60 87.66 803 0 28 59,467,000 116,590,000 0.58 11.11
P09496 Clathrin light chain A CLTA 5 16.10 27.08 248 0 7 508,650,000 765,700,000 0.58 33.33
R4GMU1 GDH/6PGL endoplasmic bifunctional protein H6PD 2 3.70 90.17 802 0 4 20,789,000 34,525,000 0.60 9.09
Q8NCN5 Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial PDPR 3 3.40 99.36 879 0.0012863 3 10,312,000 28,116,000 0.60 4.81
Q8WY22 BRI3-binding protein BRI3BP 1 7.60 27.84 251 0.0081094 2 5,099,700 6,146,700 0.62 NaN
Q6IB29 Probable rRNA-processing protein EBP2 EBNA1BP2 2 11.10 34.85 306 0 6 30,468,000 61,825,000 0.62 6.10
O15031 Plexin-B2 PLXNB2 10 6.50 205.12 1838 0 18 30,986,000 221,240,000 0.62 36.21
C9JYQ9 60S ribosomal protein L22-like 1 RPL22L1 2 19.80 14.48 121 0 6 616,860,000 1,011,100,000 0.66 15.31
Q9UNS2 COP9 signalosome complex subunit 3 COPS3 5 17.30 47.87 423 0 56 157,900,000 294,180,000 0.66 42.51
A0A0G2JNZ5 Glucosylceramidase GBA 3 7.60 50.26 449 0 6 118,570,000 187,510,000 0.67 17.54
A0A140VK17 EH domain-binding protein 1 EHBP1 2 2.80 132.29 1160 0 4 6,547,700 17,018,000 0.71 NaN
F5GWG3 Retinoic acid-induced protein 3 GPRC5A 1 4.80 30.52 273 0 11 149,190,000 185,790,000 0.76 18.13
S4R3V8 Lipolysis-stimulated lipoprotein receptor LSR 2 7.20 66.18 601 0 4 26,182,000 34,332,000 1.05 NaN
Q9NWT6 Hypoxia-inducible factor 1-alpha inhibitor HIF1AN 3 12.00 40.29 349 0 17 0 46,940,000 NaN NaN
B7ZBQ1 Mediator of RNA polymerase II transcription subunit 20 MED20 1 18.30 6.71 60 0.0087397 2 0 18,220,000 NaN NaN
H3BR38 Target of rapamycin complex subunit LST8 MLST8 1 25.00 7.69 72 0.00052938 3 0 13,473,000 NaN NaN
P52815 39S ribosomal protein L12, mitochondrial MRPL12 1 35.40 21.35 198 0.005217 2 0 0 NaN NaN
A0A024R1I3 Pyridoxal phosphate phosphatase PDXP 2 14.50 31.70 296 0 6 0 40,714,000 NaN NaN
A0A2R8YDS2 Ras/Rap GTPase-activating protein SynGAP SYNGAP1 2 2.00 137.26 1249 0.0045227 2 0 18,400,000 NaN NaN
J3KQA0 Synaptotagmin-1 SYT1 3 18.10 47.26 419 0 26 0 339,450,000 NaN NaN
Q5W0C6 Torsin-3A TOR3A 1 8.60 20.06 174 0.0015023 3 0 16,493,000 NaN NaN
B4DSK7 Mediator of RNA polymerase II transcription subunit 1 MED1 1 1.20 140.16 1330 0.0029297 2 0 14,356,000 NaN NaN
Q99549 M-phase phosphoprotein 8 MPHOSPH8 1 2.70 69.24 602 0 12 0 15,782,000 NaN NaN