Fig. 5.

DIM inhibits ESCC growth in an AHR-dependent manner. a CCK8 assay indicated that DIM inhibited cell viability through the time-dependent and dose-dependent manner. b An antagonist of AHR, Resveratrol (RSV, 0, 1, 5, 10, 20 μmol/L), was added in medium with DIM 40 μmol/L for 6 h to reverse cell viability partially. c Western blot visualized the corresponding protein expression change after RSV treatment with DIM. d Colony formation visualized that DIM inhibited cell growth. e Proper incubation time for DIM treatment was selected by western blot. The 48-h time point was applied in next assays. *, P < 0.05; **, P < 0.01; ***, P < 0.001; N.S, no significance