Fig. 5.

TP73-AS1 decoyed miR-874-3p to promote TFAP2B-mediated proliferation, migration, invasion and EMT in RB cells. A Sequence alignment of TP73-AS1 with potential wild type and mutant type of miR-874-3p targeting sites. B-C EGFP reporter assay showed that TP73-AS1 is a target of miR-874-3p. D-E Y79 and HXO-RB44 cellular viabilities were confirmed by MTT assay. F Colony formation ability of Y79 and HXO-RB44 cells were detected by colony formation assay. G-H The migration and invasion ability of Y79 and HXO-RB44 cells were confirmed by transwell assays. I E-cadherin, cytokeratin and Vimentin protein levels in Y79 and HXO-RB44 cells were confirmed by Western blot assays