Fig. 6.

TP73-AS1 interacted with miR-874-3p to facilitate RB tumorigenesis through Wnt/β-catenin Pathway. A Luciferase reporter assays. B TOP-flash assay shown that the rescue effect of TFAP2B knockdown on WNT/β-catenin pathway caused by TP73-AS1. C-F RT-qPCR and Western blot analysis showed that TP73-AS1 and miR-874-3p could regulate the expression levels of TFAP2B and protein levels of β-catenin downstream targets. G-H Western blot analysis shown that cytoplasm or nuclear β-catenin protein levels in the Y79 or HXO-RB44 cells. I Proposed model showing that TP73-AS1 promotes TFAP2B over-expression by interacting with miR-874-3p and promoting WNT/β-catenin pathway activation to promote tumorigenesis