Table 2.

Hspa13 mRNA was increased in LPS-induced PBs/PCs.

	Gene symbol	Affymetrix microarray			RNA-sequencing
		Fold change		Regulation	None	LPS	The ratio of LPS to none
		1st	2nd
B-cell marker	Cd19	0.21	0.32	Down	20349.00	15874	0.78
	Ms4a1	0.43	0.52	Down	25484.96	15411	0.60
GC B cell marker	Pax5	0.09	0.29	Down	30458.14	11625	0.38
	Bcl6	0.16	0.17	Down	1865.00	403	0.22
PB/PC marker	Prdm1	34.05	27.52	Up	244.00	6055	24.82
	Xbp1	10.17	8.79	Up	2008.00	15347	7.64
	Hspa13	9.23	8.18	Up	430.00	1907	4.43

The splenocytes were separated from three C57BL/6 mice per group. B cells were sorted by B220 microbeads and stimulated for 3 days in vitro by 10 μg/ml LPS. The transcripts in B cells and LPS-stimulated B cells were determined by Affymetrix Microarray and RNA-sequencing. The fold change of B cell-associated genes including CD19 and Ms4a1 (CD20), GC B cell-associated genes including Pax5 and Bcl6, PB/PC-promoting genes including Prdm1 and Xbp1, and the interested gene Hspa13 in LPS-stimulated group to unstimulated group is shown. 1st and 2nd represent 2 independent experiments. Total exon fragments (values) of Cd19, Ms4a1, Pax5, Bcl6, Prdm1, Xbp1, and Hspa13 in B cells (None) and LPS-stimulated B cells (LPS) are also shown.