Table 3.

Hspa13 mRNA was expressed in PBs.

	Cell numbers	Hspa13+ cells	% Hspa13+ cells	Hspa13 relative expression
Immature B cells	14	2	14.29	0.11
Mature B cells	16	0	0.00	0.00
Memory B cells	13	0	0.00	0.00
GC B cells	34	4	11.76	0.07
PBs	10	10	100.00	0.59

For sheep red cell (SRC) immunization, three 9-week-old C57BL/6 mice were i.p. injected with 1 x 10⁹ SRC and were sacrificed on day 14. Splenic B cells were sorted using B220 microbeads. The transcripts in B cells were determined by 10x Genomics and single-cell RNA-sequencing. 14 immature B cells (Cd3d⁻Cd3e⁻Cd3g⁻Cd4⁻Cd8a⁻Cd19⁺Ptprc⁺Ms4a1⁺Ighm⁺Ighd⁻Ighg1⁻Ighg2b⁻Ighg2c⁻Ighg3⁻Igha⁻Ighe⁻Bcl6⁻Aicda⁻Prdm1⁻Xbp1⁻Sdc1⁻), 16 mature B cells (Cd3d⁻Cd3e⁻Cd3g⁻Cd4⁻Cd8a⁻Cd19⁺Ptprc⁺Ms4a1⁺Ighm⁺Ighd⁺Ighg1⁻Ighg2b⁻Ighg2c⁻Ighg3⁻Igha⁻Ighe⁻Bcl6⁻Aicda⁻Prdm1⁻Xbp1⁻Sdc1⁻), 13 memory B cells (Ighg1⁺,Ighg2b⁺,Ighg2c⁺,Ighg3⁺, Igha⁺, or Ighe⁺ Cd3d⁻Cd3e⁻Cd3g⁻Cd4⁻Cd8a⁻Cd19⁺Ptprc⁺Ms4a1⁺Ighd⁻Bcl6⁻Aicda⁻ Prdm1⁻Xbp1⁻Sdc1⁻),34 GC B cells (Cd3d⁻Cd3e⁻Cd3g⁻Cd4⁻Cd8a⁻Cd19⁺Ptprc⁺Ms4a1⁺ Ighm⁺Ighd⁻Pax5⁺Bcl6⁺Aicda⁺Prdm1⁻Xbp1⁻Sdc1⁻), 10 PBs (Ighm⁺,Ighg1⁺,Ighg2b⁺,Ighg2c⁺,Ighg3⁺,Igha⁺, or Ighe⁺ Cd3d⁻Cd3e⁻Cd3g⁻Cd4⁻Cd8a⁻Cd19⁺Ptprc⁺Ms4a1⁺ Ighd⁻Bcl6⁻Aicda⁻Prdm1⁺Xbp1⁺Sdc1⁺) were chosen and Hspa13 expression was compared in these cells.