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. 2020 Jun 4;10:9145. doi: 10.1038/s41598-020-65973-3

Figure 4.

Figure 4

Determination of the KD of the aptamers R10C5 (a) and R10C1 (b). Lp (lp120292) was incubated with 10-fold dilutions of the FITC-tagged aptamers and the fluorescence was measured by flow cytometry. The number of cells displaying fluorescence above the autofluorescence were counted as bound cells, as described in Fig. 2. The graphs show individual values of three experiments. The equilibrium dissociation constant, KD was calculated using GraphPad Prism 7.03. The non-linear regression (solid line) with 95% confidence interval error envelopes (dotted lines) is shown.