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. 2020 Jun 4;10:9145. doi: 10.1038/s41598-020-65973-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The specificity of FITC-labelled R10C5 and R10C1 aptamers binding to Lp strain lp120292 (positive control), to counter SELEX Pseudomonas strains (a,b) as well as to environmental isolates (c,d) was analyzed by flow cytometry. The binding of R10C5 to Lp strain Lp02 was also analyzed. The percentage of cells bound by R10C5 (a,c) and R10C1 (b,d) to counter-SELEX strains (a,b) and environmental isolates (c,d) are presented. The binding of a scrambled sequence of aptamer R10C5 and a scrambled sequence of aptamer R10C1 to Lp strain lp120292 was also investigated (scrambled). The values of three experiments are shown with the mean and standard deviation. A one-way ANOVA with a Dunnett correction for multiple comparisons was used to infer statistical significance compared to Lp strain lp120292: ***P < 0.001; ns, not significant.