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. 2020 Jun 4;11:2809. doi: 10.1038/s41467-020-16580-3

Fig. 3. Tau phase separates into liquid droplets in multiple buffers.

Fig. 3

a Tau-GFP (2 µM) forms liquid droplet in the LLPS buffer, tris buffered saline, phosphate-buffered saline and X/2 buffer under crowding conditions, not in the absence of crowding. Scale bar is 5 µm. b The extent of droplet formation was significantly higher in LLPS and PBS buffers compared to X/2 buffer (graph represents mean ± SD; n = 3 independent experiments; ****p < 0.001, ***p = 0.0005, **p = 0.0098, *p = 0.0109, two-way ANOVA with Holm–Sidak post-hoc test). c Tau-GFP (2 µM) forms liquid droplets under crowding conditions (+PEG) across the range of physiological levels of ATP (0–8 mM) in the LLPS buffer. Scale bar is 5 µm. d ATP (0–8 mM) does not significantly impact the amount of tau-GFP droplet formation (graph represents mean ± SD; n = 3 independent experiments; p = 0.095, one-way ANOVA). Source data for b and d provided in the Source Data file.