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. 2020 Jun 4;11:2812. doi: 10.1038/s41467-020-16632-8

Fig. 5. AID-dependent depletion of REV7-deficient GC B cells.

Fig. 5

Fractions of GC B cells (GL7+Fas+) from spleens (a) and Peyer’s patches (b) are showed with exampled flow cytometry plots and summary bar graphs. Each dot indicate result from an individual mouse. Six Cd19cre and seven Cd19creRev7fl/fl mice were assayed for a and b. c Immunohistochemistry of spleens of indicated genotype. Representative images from three sets of mice are showed. Scale bar, 500 μm, is indicated as a black line on each picture. d Genotyping of indicated B (B220+) cell populations (PNAlow: naive B cell, PNAhigh: GC B cell) in the spleen and Peyer’s patch. n = 5 mice. AID deficiency fully rescued the GC B-cell depletion in spleens (e) and Peyer’s patches (f) of Rev7-deficient mice. Four Cd19cre and five Cd19creRev7fl/fl mice were assayed for e. Six Cd19cre and Cd19creRev7fl/fl mice were assayed for f. Data are represented as mean ± SD. Two-tailed unpaired t-test was performed for a, b, e, and f; two-tailed paired t-test was performed for d. **p < 0.01, ns: p > 0.05. P-values and sample sizes are provided in Supplementary Table 2. Source data are provided as a Source Data file.