Fig. 2. RTS,S/AS01_E booster and long-term immunogenicity against vaccine antigens: IgG3-4 subclasses and IgM for CSP constructs and HBsAg at month (M) 20, 21, and 32 for RTS,S/AS01 vaccinees with (R3R) and without (R3C) booster, and comparator (C3C).

Boxplots with medians, interquartile ranges (IQR), upper whisker as the smallest between maximum × value and Q3 + 1.5*IQR, lower whisker as the largest between minimum × value and Q1 – 1.5*IQR, and log₁₀(geometric mean(MFI)) (diamond). Non-parametric tests were used to compare the booster response (M20 vs. M21) and the long-term immunogenicity (M21 vs. M32), as well as to compare the R3C and R3R groups at each timepoint. Only p-values < 0.05 after adjustment for multiple testing are shown. The y-axis is in logarithm 10 scale. R3R (green): three doses of RTS,S/AS01_E and a RTS,S/AS01_E booster. R3C (red): three doses of RTS,S/AS01E and a comparator booster. C3C (blue): three doses of a comparator vaccine and a comparator booster.