FIGURE 3.

α-Syn-112 exhibits enhanced binding to anionic phospholipids. (A) Representative Western blots showing α-syn-140 and α-syn-112 binding to liposomes containing 5% PS, PI, PI(3)P, PI(4)P, PI(4,5)P₂, and PI(3,4,5)P₃. Red box indicates liposome-containing fractions. α-Syn-112 bound with increased efficacy to all liposomes containing anionic phospholipids. (B) Quantification of lipid bound protein show enhanced binding to all anionic phospholipids tested. (PS = α-syn-140: 34.42 ± 10.07, n = 5; α-syn-112: 71.50 ± 2.76, n = 5; p = 0.0038) (PI = α-syn-140: 24.90 ± 10.73, n = 3; α-syn-112: 71.78 ± 5.91, n = 3; p = 0.0187) (PI(3)P = α-syn-140: 22.83 ± 9.1, n = 3; α-syn-112: 66.86 ± 3.78, n = 4; p = 0.0014) (PI(4)P = α-syn-140: 20.91 ± 6.05, n = 3; α-syn-112: 57.07 ± 8.83, n = 4; p = 0.0264) (PI(4,5)P₂ = α-syn-140: 43.68 ± 3.00, n = 4; α-syn-112: 67.50 ± 2.48, n = 4; p = 0.0009) (PI(3,4,5)P₃ = α-syn-140: 35.02 ± 1.457, n = 3; α-syn-112: 87.84 ± 6.33, n = 3; p = 0.0012). Data points indicate mean ± SEM. Asterisks indicate statistical significance by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.