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. 2020 May 29;8:537. doi: 10.3389/fbioe.2020.00537

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Copyright © 2020 Zhang, Cao, Liang, Tan, Luo, Xu and Saw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of macrophages treated with differently charged SPIONs on tumor cells. (A) Sketches of chemotaxis effect on macrophage incubated with tumor cells. (B) Percentage of macrophages on lower chamber in different groups. ***P < 0.005, compared with control (cell + macrophage). (C) The fluorescence images of DID-labeled macrophages (red) in the lower chamber. Scale bar, 1000 μm. (D) Co-incubation of macrophage and tumor cells with 0.4 μm trans well. (E) Calculation of Fenton positive tumor cells of different groups in lower chamber. ***P < 0.005, compared with control (cell + macrophage). (F) Fluorescence images of Caspase 9 (yellow) expression in tumor cells (green). Scale bar, 200 μm.