Fig. 2.

Reduced preweaning survival of Agt^Agrp-KO mice [mice exhibiting selective deletion of the angiotensinogen (Agt) gene in cells that express agouti-related peptide (Agrp)]. A: total live mice at weaning (postnatal day 21, n = 139 mice) from the Agt^Lepr-KO colony [mice exhibiting selective deletion of the Agt gene in cells that express leptin receptor (Lepr)] illustrates observation of expected 25% survival of Agt^Lepr-KO mice (i.e., n = 35 mice expected, and 38 mice observed). B: distribution of live Agt^Lepr-KO and littermate control male (n = 72) vs. female (n = 67) mice from A. C: total live mice at weaning (postnatal day 21, n = 210 mice) from the Agt^Agrp-KO colony illustrates significant deviation from expected 25% survival of Agt^Agrp-KO mice due to preweaning lethality (i.e., n = 53 mice expected, and only 28 mice observed). D: breakdown of live Agt^Agrp-KO and littermate control male (n = 99) vs. female (n = 111) mice from C highlights more penetrant lethal phenotype in male Agt^Agrp-KO mice (i.e., n = 25 mice expected, and only n = 10 mice observed) than female Agt^Agrp-KO mice (n = 28 mice expected, and 18 mice observed).