Fig. 5.
Altered adrenal expression of angiotensinogen (Agt) in adult AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express agouti-related peptide (Agrp)]. A: plasma AGT protein; n = 8 mice each. B: Agt mRNA in liver, kidney, and adrenal glands; n = 7–8 mice each. C: urine production rate. D and E: concentration of corticosterone and aldosterone in urine; n = 10–12 mice each. F and G: total daily elimination of corticosterone and aldosterone to urine. For all panels, open circles indicate male mice. Summary data are presented as means ± SE. *P < 0.05 by two-tailed independent t test. H: PCR amplification of the Agt locus from various tissues in AgtAgrp-KO mice. Intact amplicon, 2,009 bp, vs. recombined amplicon, 337 bp.