Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.