Table 1.
Primers for quantitative PCR
| Gene | Forward Primer | Reverse Primer | TaqMan Probe |
|---|---|---|---|
| Agt | Mm00599662_m1 | ||
| Ren | Mm02342888_gH | ||
| Actb | Mm02619580_g1 | ||
| Slc12a3 (NCC) | 5-AAGTCGGGTGGCACCTATTTCCTT-3 | 5-TTACGGTTTCTGCAAAGCCCACAG-3 | |
| Slc9a3 (NHE3) | 5-TCCTCTCAGCCATTGAGGACATCT-3 | 5-ACTTTGCTGAGGAACTTCCGGTCA-3 | |
| Slc12a1 (NKCC2) | 5-CCATGGTAACCTCTATCACTGGGT-3 | 5-TCAAGCCTATTGACCCACCGAACT-3 | |
| Scnn1a (ENaCα) | 5-ACAATGGTTTGTCCCTGACACTGC-3 | 5-TCACGTTGAAGCCACCATCATCCA-3 | |
| Scnn1b (ENaCβ) | 5-TCTGCCAACCCTGGGACTGAATTT-3 | 5-TGGCATAGATGCCCTCCTCTCTAA-3 | |
| Scnn1g (ENaCγ) | 5-GCCAATCAGTGTGCAAGCAATCCT-3 | 5-TTATTTGCTGGCTTTGGTCCCAGG-3 | |
| Atp1a1 (Na/K ATPase) | 5-TGAAGCTGACACCACGGAGAATCA-3 | 5-TGCCGCTTAAGAATAGGCAGGTT-3 |
Agt, angiotensinogen.