TABLE 1.
Bacterial strains and plasmids used in this study.
| Bacterial strain or plasmid | Relevant characteristics/sequence | Source |
| E. coli strains | ||
| DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk–, mk+) phoA supE44 λ– thi–1 gyrA96 relA1 | Invitrogen |
| SM10-λ pir | Mobilizing strain, RP4 integrated in the chromosome; Knr | Simon et al., 1983 |
| P. aeruginosa strains | ||
| PAO1 | Wild type, lab strain | Holloway et al., 1994 |
| PAO1(ΔclpV3) | ClpV3 knockout mutant of PAO1 | This study |
| Plasmids | ||
| pMS402 | Expression reporter plasmid carrying the promoterless luxCDABE; Kmr Tmpr | Duan et al., 2003 |
| CTX-6.1 | Integration plasmid origins of plasmid mini-CTX-lux; Tcr | Kong et al., 2013 |
| pRK2013 | Broad-host-range helper vector; Tra+, Kmr | Ditta et al., 1980a |
| pEX18Tc | oriT+ sacB+ gene replacement vector with multiple-cloning site from pUC18; Tcr | Hoang et al., 1998 |
| pAK1900 | E. coli-P. aeruginosa shuttle cloning vector, Ampr | Sharp et al., 1996 |
| pEX18Tc-clpV3up | pEX18Tc carrying the upstream fragment of clpV3 | This study |
| pEX18Tc-clpV3up+dw | pEX18Tc carrying the upstream and downstream fragment of clpV3 | This study |
| pAK-clpV3 | pAK1900 with a 2774 bp fragment of clpV3 between KpnI and HindIII; Ampr, Cbr | This study |
| CTX-phzA1 | Integration plasmid, CTX6.1 with a fragment of pKD-phzA1 containing phzA1 promoter region and luxCDABE gene; Kmr, Tmpr, Tcr | Guo et al., 2016 |
| CTX-phzA2 | Integration plasmid, CTX6.1 with a fragment of pKD-phzA2 containing phzA2 promoter region and luxCDABE gene; Kmr, Tmpr, Tcr | Guo et al., 2016 |
| pKD -exoY | pMS402 containing exoY promoter region, Kmr, Tmpr | Guo et al., 2016 |
| pKD -exsD | pMS402 containing exsD promoter region, Kmr, Tmpr | Kong et al., 2013 |
| pKD-cdrA | pMS402 containing cdrA promoter region, Kmr, Tmpr | Bhagirath et al., 2018 |