Table 2. Results of Jamestown Canyon virus and other arbovirus tests from the patients described, blanks indicate no testing.
| Assay | Sample | Patient 1 | Patient 2 | Patient 3 | Patient 4 | Patient 5 | Patient 6 | Patient 7 | Patient 8 | Patient 9 |
|---|---|---|---|---|---|---|---|---|---|---|
| Time since symptom onset | < 1week | 3 weeks | 10 weeks | 3 days | 3 weeks | 5 months | <1 week | 2 weeks | <1 week | |
| JCV* IgM ELISA† | Serum | + | + | + | + | + | + | + | + | |
| JCV PRNT† | Serum | 1:160 | 1:1280 | 1:320 | 1:160 | 1:80 | 1:2560 | 1:160 | 1:160 | |
| JCV IgM ELISA | CSF* | + | – | |||||||
| JCV PRNT | CSF | 1:1280 | – | |||||||
| WNV* IgM EIA† | Serum | – | – | – | – | – | Indeterminate | – | – | – |
| WNV PRNT | Serum | – | – | |||||||
| WNV IgM EIA | CSF | – | – | – | – | |||||
| EEE* IgM EIA | Serum | – | – | – | – | – | – | – | ||
| EEE IgM EIA | CSF | – | – | – | ||||||
| LACV* ELISA | Serum | + | + | Equivocal | – | |||||
| LACV PRNT | Serum | 1:160 | 1:160 | 1:20 | 1:40 | – | ||||
| Powassan IgM ELISA | Serum | – | – | – | – | – | – | – | ||
| Powassan IgM ELISA | CSF | – | ||||||||
| SLEV* IgM IF† | Serum | ≥1:10‡ | ||||||||
| SLEV IgG IF | Serum | – | ||||||||
| SLEV/WNV IgM MIA† | – |
*JCV = Jamestown Canyon virus, WNV = West Nile virus, EEE = Eastern equine encephalitis virus, LACV = La Crosse virus, SLEV = Saint Louis encephalitis virus, CSF = cerebrospinal fluid.
†ELISA = enzyme-linked immunosorbent assay, PRNT = plaque reduction neutralization test, EIA = enzyme immunoassay, IF = immunofluorescence, MIA = microsphere immunoassay.
‡This finding was not replicated on confirmatory testing at the CDC, so likely represents a false-positive.