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[Preprint]. 2020 May 8:2020.05.04.20091231. [Version 1] doi: 10.1101/2020.05.04.20091231

Figure 2: Development of STOPCovid using RT-LAMP and thermophilic AapCas12b.

Figure 2:

A. Comparison of the STOPCovid N gene LAMP primer set to two established LAMP primer sets measured by real-time fluorescence across a range of concentrations of SARS-CoV-2 genomic standards. NTC, no template control.

B. Temperature dependence of AapCas12b collateral activity when incubated with RT-LAMP amplified 20 fM SARS-CoV-2 genomic standard (blue) or no template control, NTC (gray). Collateral activity was measured as 5-minute end point fluorescence after incubation.

C. Comparison of AapCas12b collateral activity with either a previously published sgRNA scaffold (Teng et al., 2019) (blue) or an AacCas12b-based scaffold (red) when incubated with RT-LAMP amplified 20 fM SARS-CoV-2 genomic standard.

D. Collateral activity of AapCas12b using different guides when incubated with RT-LAMP amplified 20 fM SARS-CoV-2 genomic standard.